The Effect of Human Placental Extracts in Suppressing Tumor Cell and Preventing Normal Cell Damage / 日本補完代替医療学会誌

Objectives: To analyze the effect of human placental extracts (HPEx) on hepatocellular carcinoma cells in vitro. Methods: The hepatocellular carcinoma cell lines, namely, HLE and Huh-7, were used. The cells were subjected to a growth assay using the formazan dye method; the effect of combination treatment with sorafenib and HPEx was also assessed. The preventing normal cell damage effect of HPEx was analyzed by virtual therapy where possible; the experimental protocol was constructed on the basis of pre- and post-sorafenib treatment data. Cytotoxicity was measured by lactate dehydrogenase (LDH) assay. Results: HPEx caused significant dose-dependent suppression in the growth of HLE and Huh-7 cells. These tumor cells were significantly suppressed by combination treatment with HPEx and sorafenib. In addition, HPEx potentiated sorafenib sensitivity against tumor cells, and significantly prevented sorafenib-induced cytotoxicity in primary cultured rat hepatocytes under all designed experimental conditions. Specifically, pre-treatment with HPEx had a greater effect than post-treatment with HPEx. Conclusion: HPEx suppresses tumor cell growth, potentiates sorafenib efficacy, and has a preventing normal cell damage effect; this triple functionality of HPEx makes it a useful agent for liver cancer therapy.

Neutrophilic Eccrine Hidradenitis Probably Induced by Intravenous Administration of Placental Extracts / 대한피부과학회지

Neutrophilic eccrine hidradenitis (NEH) is a rare, self-limited, neutrophilic dermatosis characterized histologically by a neutrophilic infiltration around the eccrine coils and necrosis of the eccrine glands. It occurs most commonly in patients undergoing chemotherapy for acute myelogenous leukemia and other malignant disease, but, other associations, such as infections, drugs or even in generally healthy individuals. We report a case of 45-year-old Korean woman with numerous erythematous papules on her trunk after treatment with intravenous administration of placental extracts. The hisopathological findings were compatible with neutrophilic eccrine hidradenitis.

The Effect of Human Placental Lipopolysaccharide and Protein-free Serum on Hematopoietic Reconstitution in Normal and Irradiated Mice / 第二军医大学学报

In the present study, the contents of pluripotential stem cell (CFU-S) and granulopoietic progenitor (CFU-GM) in bone marrow, and morphological characteristics of hematopoietic tissue were tested at different time after injection of human placental lipopolysaccharide or protein-free serum in normal and ?-ray-irradiated mice. Our study showed that both of these placental extracts could not only stimulate hematopoiesis in normal mice but also accelerate hematopoietic reconstitution, and increase the survival of mice exposed to 7.5 Gy. From these results, we consider that administration of these extracts might be useful in accelerating hematopoietic recovery in patients with chemotherapy or radiotherapy induced neutropenia.